Restriction Map Generator

This tool analyzes a DNA sequence to identify Restriction Enzyme Sites and generate a comprehensive map overview of their locations within the DNA sequence. Enter a DNA sequence in the box below to analyze the sequence for restriction sites and generate a restriction map. The restriction enzymes used in the analysis are listed at the bottom of this page.

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Restriction Enzyme Preferences

  • Use all restriction enzymes.
  • 6 Cutters only
  • Choose from this list of enzymes:

Restriction Map Preferences

  • Show unique sites only
  • Show all restriction sites

The following restriction enzymes are available with this tool:

AatII (gacgtc)

AccI (gtmkac)

AflII (cttaag)

AflIII (acrygt)

AhaII (grcgyc)

AluI (AGCT)

AlwI (ggatc)

AlwNI (cagnnnctg)

ApaI (gggccc)

ApaLI (gtgcac)

AseI (attaat)

Asp718 (ggtacc)

AvaI (cycgrg)

AvaII (ggwcc)

AvrII (cctagg)

BamHI (ggatcc)

BanI (ggyrcc)

BanII (grgcyc)

BbeI (ggcgcc)

BbvI (gcagc)

BbvII (gaagac)

BclI (tgatca)

BcnI (ccsgg)

BglI (gccn5ggc)

BglII (agatct)

BsaAI (yacgtr)

BsmAI (gtctc)

Bsp1286I (gdgchc)

BspHI (tcatga)

BspMI (acctgc)

BspMII (tccgga)

BssHII (gcgcgc)

BstBI (ttcgaa)

BstEII (ggtnacc)

BstNI (ccwgg)

BstUI (cgcg)

BstXI (ccan6tgg)

BstYI (rgatcy)

Bsu36I (cctnagg)

Cfr10I (rccggy)

ClaI (atcgat)

DdeI (ctnag)

DpnI (gatc)

DraI (tttaaa)

DraIII (cacnnngtg)

DrdI (gacn6gtc)

DsaI (ccrygg)

EaeI (yggccr)

EagI (cggccg)

EarI (ctcttc)

Eco47III (agcgct)

Eco57I (ctgaag)

EcoNI (cctn5agg)

Eco0109I (rggnccy)

EcoRI (gaattc)

EcoRII (ccwgg)

EcoRV (gatatc)

EspI (gctnagc)

Fnu4HI (gcngc)

FokI (ggatg)

FspI (tgcgca)

GsuI (ctggag)

HaeI (wggccw)

HaeII (rgcgcy)

HaeIII (ggcc)

HgaI (gacgc)

HgiAI (gwgcwc)

HgiEII (accn6ggt)

HhaI (gcgc)

HinCII (gtyrac)

HindIII (aagctt)

HinfI (gantc)

HinPI (gcgc)

HpaI (gttaac)

HpaII (ccgg)

HphI (ggtga)

KpnI (ggtacc)

MaeI (ctag)

MaeII (acgt)

MaeIII (gtnac)

MboI (gatc)

MboII (gaaga)

MluI (acgcgt)

MmeI (tccrac)

MnlI (cctc)

MscI (tggcca)

MseI (ttaa)

MspI (ccgg)

NaeI (gccggc)

NarI (ggcgcc)

NciI (ccsgg)

NcoI (ccatgg)

NdeI (catatg)

NheI (gctagc)

NlaIII (catg)

NlaIV (ggnncc)

NotI (gcggccgc)

NruI (tcgcga)

NsiI (atgcat)

Nsp7524I (rcatgy)

NspBII (cmgckg)

NspHI (rcatgy)

PaeR7I (ctcgag)

PflMI (ccan5tgg)

PleI (gagtc)

PmlI (cacgtg)

PpuMI (rggwccy)

PstI (ctgcag)

PvuI (cgatcg)

PvuII (cagctg)

RsaI (gtac)

RsrII (cggwccg)

SacI (gagctc)

SacII (ccgcgg)

SalI (gtcgac)

Sau3AI (gatc)

Sau96I (ggncc)

ScaI (agtact)

ScrFI (ccngg)

SecI (ccnngg)

SfaNI (gcatc)

SfiI (ggccn5ggcc)

SmaI (cccggg)

SnaBI (tacgta)

SpeI (actagt)

SphI (gcatgc)

SplI (cgtacg)

SspI (aatatt)

StuI (aggcct)

StyI (ccwwgg)

TaqI (tcga)

Tth111I (gacnnngtc)

Tth111II (caarca)

XbaI (tctaga)

XcaI (gtatac)

XhoI (ctcgag)

XcmI (ccan9tgg)

XmaI (cccggg)

XmnI (gaan4ttc)


© 2008-2017 Greg Cope